PENGERTIAN SKRINING FITOKIMIA PDF DOWNLOAD

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skrining fitokimia herba pacar airmengandung senyawa polifenol, tannin galat, flavonoid, kuinon . Penetapan bobot jenis dilakukan menggunakan ekstrak 1%. SKRINING FITOKIMIA - Download as Powerpoint Presentation .ppt /.pptx), PDF Download as PPTX, PDF, TXT or read online from Scribd . PENGERTIAN. PENGERTIAN SKRINING FITOKIMIA PDF DOWNLOAD.


Pengertian Skrining Fitokimia Pdf Download

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Menentukan cara penapisan. Pengertian Skrining Fitokimia PDF – Author: Cole Eileen Country: China Language: English (Spanish) Genre: Spiritual Published. Tujuan penelitian adalah untuk mengetahui skrining fitokimia, karakterisasi simplisia, kandungan total fenol dan uji Download full-text PDF. Download Citation on ResearchGate | KAJIAN FITOKIMIA EKSTRAK KULIT BIJI Hasil penelitian menunjukkan bahwa berdasarkan skrining fitokimia, kulit biji.

Therefore, plant materials are considered to be the alternative sources for finding out new leads for 2. Bauhinia vahlii[9] is a gigantic climbing ever green tree S wiss albino mice 20 - 25 g and M ale W istar rats and largest creeper in India and can grow up to g were downloadd from the animal house of Gosh m long. The woody stem can get as thick as 20 cm. The enterprises, Kolkata and housed in polypropylene cages spreading stout branches are covered with rusty fine hair.

Prior to the Branchlets[10] densely pubescent and terminating in a pair experiments, mice and rats were fed with standard diet for of revolute tendrils; young branches, tendrils, petioles, 1 week in order to adapt to laboratory conditions. They were underside of leaves especially along the nerves and fasted over night but allowed free access to water before the inflorescence clothed with dense ferruginous tomentum.

SKRINING FITOKIMIA

The experimental protocols were approved by Leaves [11] very variable in size, often up to 18 in. Preparation of plant material lobes obtuse, rounded; petiole in. Flowers[11] white, on long slender pedicles, in terminal corymbose or The whole plant was first sun dried for several weeks, corymbose racemes.

Then the crushed parts Fruit [12] is a flat woody pod with fine rusty hairs, of the plant were ground into coarse powder with the help cm long. The plant has been reported to have agathisf of a mechanical grinder. By using the concept of the nature lavone,flavonoid,betulinic acid,triterpene,campesterol of solubility and distribution of the active ingredients, and steroid in leaves [13] , catechin,gallic acid methyl powdered material 1 g was packed in soxhlet apparatus ester,benzenoid,mopanol and 4-O-methyl ester[13].

The [17] and extracted successively with Pet. Ether , to plant is also reported to contain kaemferol, quercetin, rutin defat the drug, petroleum ether was removed from the , betulinic acid[14] and Quercitrin, Stigmasterol, Sitosterol[16] powdered defatted drug which was then extracted with in leaves. The whole each mixture then underwent filtration inflammatory, anti-diabetic activity of Bauhinia vahlii stem through what man filter paper.

The filtrates Pet. Ether, bark extracts using carrageenan induced rat paw edema Benzene, Chloroform and Ethanol filtrate obtained were and Streptozotocin induced diabetic.

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It rendered a gummy concentrates. The gummy phytochemical screening. Materials and methods 2. Phytochemical screening 2. Himedia laboratories, Mumbai, India. All other chemicals and solvents Pet. Acute toxicity study [] was performed according to Organisation for Economic Co-operation and Development 2.

Collection and identification of plant material was performed as per Karber s method. Swiss albino mice of either sex weighing between g were divided into The Plant Bauhinia vahlii were collected in the month of six groups with six animals each. The determine LD50 of various extracts of Bauhinia vahlii. The plant material was taxonomically identified by Dr. The extracts were administered orally at Sambalpur, Odisha.

The LD50 values of various extracts are 2. A nti-inflammatory activity was evaluated using the 2. Experimental design Carrageenan induced rat paw oedema according to the technique of Winter et al [].

The animals were housed Thirty-Six experimental animals were randomly selected in cages under standard laboratory condition. They had and divided into six groups denoted as Group I, Group II, free access to standard diet and water.

Each group received a particular h before the experiment. The initial right hind paw volume treatment i.

Prior to any treatment, each rat was weighed properly 0. The hind paw Wistar rats in each group.

Each group received a particular volume was measured plethysmometrically before and after treatment i. Prior to any treatment, each 2. Induction of diabetes in rats [] Rats were made diabetic with an intraperitoneal injection 2. Diabetes was confirmed in STZ The anti-inflammatory activity of Bauhinia vahlii was rats by measuring the fasting blood glucose level 48 h after studied using acute carrageenan induced paw edema the injection of STZ.

Rats with blood glucose level above models of inflammation.

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Estimation of blood glucose This model is based on the principle of release of various Blood samples were collected from the tail tips at 12, 24, inflammatory mediators by carrageenan. Edema formation 36 and 48 h after streptozotacin administration and found due to carrageenan in the rat paw is biphasic event.

The that stable hyperglycemia produced after 48 h. The blood initial phase is attributed to the release of histamine sugar level was measured by digital display glucometer One and serotonin.

Initial blood sample were release of prostaglandins, protease and lysosome[24,25]. The blood glucose levels were produces inflammation resulting from plasma extravasations, measured at 2, 4, 8, 12 and 24 h after oral administration of increased tissue water and plasma protein exudation along Glibenclamide and various extracts of Bauhinia vahlii Table with neutrophil extravasations, all due to the metabolism of 3.

The first phase begins immediately after injection of carrageenan and diminishes in two hours.

The 2. Statistical Analysis second phase begins at the end of first phase and remains For Anti-inflammatory screening all experimental results through third hour up to five hours. Anti-inflammatory activity considered as statistically significant.

Antidiabetic activity 3. Result The result of antidiabetic effect depicted in Table 3. From the table it is revealed that the ethanolic and chloroform 3.

Larutan dikocok perlahan , biarkan selama beberapa menit. Steroid memberikan warna biru atau hijau, untuk Triterpenoid memberikan warna merah atau ungu Harborne, Terbentuknya warna coklat kemerahan pada antar muka lapisan menunjukkan adanya terpenoid.

Di identifikasi dengan uji Borntrager, antrakuinon berikatan dengan gula sebagai o-glikosida atau sebagai C-glikosida. Turunan antrakuinon umumnya larut dalam air panas atau dalam alkohol encer. Senyawa antrakuinon dapat bereaksi dengan basa memberikan warna ungu atau hijau Harborne, Atom nitrogen yang terdapat pada molekul alkaloid umumnya merupakan atom nitrogen sekunder ataupun tersier dan kadang terdapat sebagai atom nitrogen kuarterner Harborne, Salah satu pereaksi untuk mengidentifikasi adanya alkaloid menggunakan pereaksi Dragendorff, Wagner, dan pereaksi Mayer.

Lapisan yang memisahkan, yang terdiri atas bahan berbutir-butir fase diam , ditempatkan pada penyangga berupa pelat gelas, logam, atau lapisan yang cocok. Dari fraksi ektrak dihasilkan fraksi yang larut dalam dietil eter dan yang tidak larut dalam dietil eter. Fraksi yang tidak larut dalam dietil eter kemudian ditambahkan air sebanyak 5 mL dalam tabung reaksi dan kocok.

Ekstrak positif mengandung saponin, jika terbentuk busa dengan ketinggian cm yang bertahan selama 15 menit Harborne, Larutan dikocok perlahan , biarkan selama beberapa menit.

Steroid memberikan warna biru atau hijau, untuk Triterpenoid memberikan warna merah atau ungu Harborne, Terbentuknya warna coklat kemerahan pada antar muka lapisan menunjukkan adanya terpenoid.

Di identifikasi dengan uji Borntrager, antrakuinon berikatan dengan gula sebagai o-glikosida atau sebagai C-glikosida. Turunan antrakuinon umumnya larut dalam air panas atau dalam alkohol encer. Senyawa antrakuinon dapat bereaksi dengan basa memberikan warna ungu atau hijau Harborne, Generally, denitrification activity was the most dominant bzkteri.

Karena berfungsi sebagai penegasan, maka uji KLT hanya dilakukan untuk golongan- golongan senyawa yang menunjukkan hasil positif pada skrining fitokimia Fase diam dapat berupa serbuk halus yang berfungsi sebagai permukaan penyerap atau penyangga untuk lapisan zat cair.

Turunan antrakuinon umumnya larut dalam air panas atau dalam alkohol encer. Batang pohon dapat menebal hingga 20 cm. Anti-inflammatory activity of the leaf extacts of [25]S undhararajan R and R avichandiran V.